SCREENING STUDY OF THE PSYCHOTROPIC ACTIVITY OF NEUROPEPTIDE COMPOUNDS IN CONDITIONS OF MERCAZOLYL HYPOTHYROIDISM

Abstract

Introduction. To date, there has been an increase in thyroid diseases mediated by a violation of regulatory mechanisms on the part of the central structures of the hypothalamic-pituitary-thyroid system. Numerous scientific studies have revealed that the emerging thyroid imbalance in thyroid diseases causes the development of metabolic syndrome, disorders of the cardiovascular system, as well as neuropsychic changes, which are often the first clinical manifestations of thyroid pathology. The current direction today is the study and development of means of correction of possible neuropsychic disorders. Of interest are the compounds of the peptide structure, on the basis of which effective drugs with a wide variety of pharmacological properties are being synthesized today. It has been proven that peptide compounds have neurotropic, psychotropic, anxiolytic, and other pharmacological effects due to their effect on the functional activity of neurotransmitter systems. The aim of the study was a screening study of the psychotropic activity of peptide compounds in conditions of hypofunction of the thyroid gland. Material and methods. The study was conducted on male rats divided into groups: I-th – control animals; II-th – individuals with induced hypothyroidism; III, IV, V, VI, VII and VIII-th – animals treated with hypothyroidism, respectively, Thr-Lys-Pro-Arg-Pro-Gly-Pro, ACTH(4-7)-Pro-Gly-Pro, ACTH(6-9)-Pro-Gly-Pro, Pro-Gly-Pro, Pro-Gly-Pro-Leu, Arg-Pro-Gly-Pro, Pro-Gly-Pro-Val and Met-Thr-Lys-Pro-Arg-Pro-Gly-Pro doses 87; 87; 89; 33; 44; 42; 42; 88 mcg/kg/day, which were 1/10 of the molecular weight of the studied substances. The psychotropic activity of the studied peptides was studied using the Open Field test. Results. It was found that in the conditions of experimental hypothyroidism, there is an inhibition of motor and exploratory activity against the background of an increase in anxiety levels (duration of freezing, grooming reactions and the number of defecations). The studied peptide compounds had a corrective effect to varying degrees on the psychoemotional state of laboratory animals in conditions of hypofunction of the thyroid gland. Conclusions. The results of a screening study in a number of regulatory peptides indicate that the compounds ACTH(4-7)-Pro-Gly-Pro and ACTH(6-9)-Pro-Gly-Pro have the most pronounced psychotropic activity in relation to behavioral indicators in the Open Field test under conditions of experimental hypothyroidism.