Abstract
A lipid-requiring strain of Haliphthoros milfordensis (Oomycetes) contained no steroids when grown in the presence of either Lα-lecithin, oleic acid or 5α-cholestane. When 3-oxygenated steroids were supplied to the fungus, sterols with elongated side chains were recovered from the mycelia. 5α-Cholestan-3-one was converted to 5α-cholestan-3β-ol and 5-dihydrofucosterol. 5α-Cholestan-3β-ol was converted to 5-dihydrofucosterol. Cholesterol was converted to fucosterol. Traces of isomers of these compounds were also detected. A strain of H. milfordensis not requiring any lipid growth factor contained 24-methylenecholesterol and fucosterol. A tkinsiella dubia, a related marine oomycete not requiring any lipid growth factor, contained desmosterol, 24-methylenecholesterol and fucosterol.
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