Statistical optimization of xylanase production, using different agricultural wastes by Aspergillus oryzae MN894021, as a biological control of faba bean root diseases

Sherien M M Atalla,Nehad E Ahmed,Hassan M Awad,Nadia G El Gamal,Aliaa R El Shamy

doi:10.1186/s41938-020-00323-z

Sherien M M Atalla, Nehad E Ahmed + Show 3 more

Open Access

https://doi.org/10.1186/s41938-020-00323-z

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Abstract

BackgroundXylanase enzyme plays an important role in nature as being a part of protecting the environment from pollution. It has also various industrial applications.Main body of abstractMarine fungal isolate was recovered from red sea water at Sharm El-Sheikh province, Egypt, and tested for xylanase activity, using different agricultural wastes as a substrate. It was found that rice straw was the best substrate for xylanase production (0.37 U/ml). Thus, it was subjected for identification by 18S rDNA gene. The phylogenetic analysis results indicated that this fungal isolate belonging to Aspergillus species with a similarity of 99% and named as A. oryzae SS_RS-SH (MN894021). The regular two-level factorial design was used to optimize the important medium components, which significantly affected the xylanase production. The model in equation suggested optimal conditions of 2% of rice straw, 8 g/l of yeast extract, 4 g/l of (NH4)2SO4, 2 g/l K2HPO4, and 2.5 g/l MgSO4.7H2O for a maximum xylanase yield. The antifungal activity of crude xylanase on mycelial growth of some pathogenic fungi isolated from different hosts was investigated. The results showed that xylanase T1 had a potent antifungal activity than control. Greenhouse experiments indicated that all treatments with xylanase at different concentrations significantly decreased infection occurrence of beans, which have been effectively infected with root rot pathogens, compared to unprocessed control treatments.Short conclusionXylanase yield increased 2.43-folds than initial screening. The xylanase had a potential antifungal activity both in vitro and under greenhouse conditions. The outcome of this study ensured that this fungal strain could be used as biological control for plant disease.

Highlights

Xylanase enzyme plays an important role in nature as being a part of protecting the environment from pollution
Phylogenetic analysis and Guanine and cytosine (GC)% The phylogenetic tree (Fig. 1) disclosed that SS_RS-SH isolate was the greatest nearly correlated to A. oryzae
The GC content of the SS_RS-SH isolate was 58 mol% achieved from the phylogenetic analysis

Summary

Introduction

Xylanase enzyme plays an important role in nature as being a part of protecting the environment from pollution. It was found that rice straw was the best substrate for xylanase production (0.37 U/ml). Xylanase (E.C.2.8.1.8) is defined as a set of hemicellulose, which is necessary for the hydrolysis of 1, 4-xylans present in lignocellulostic materials (Jae et al 2009) It plays an important role in nature as being a part of protecting the environment from pollution because of its alternative way to chemical hydrolysis (El Shamy et al 2016). Fungi have been broadly used to deliver hydrolytic enzymes for industrialized applications, comprising xylanases, whose levels in fungi are commonly a lot higher than in yeast and microscopic microorganisms (Atalla et al 2020)

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