Abstract
Yarrowia lipolytica is an oleaginous yeast species with the ability to grow on a number of substrates types, especially industrial wastes. This paper concerns the statistical optimization of fermentation parameters and media to ensure consistent and improved Y. lipolytica protein production. A strain of Y. lipolytica A-101 was observed to be proficient in producing single cell protein, amino acids, and vitamin B12 while utilizing biofuel waste instead of a complete YPD medium for yeast growth. A fractional fractal design experiment was then applied, and the two fermentation parameters of temperature and pH were recognized to have a significant effect on the protein and amino acid production. Subsequently, the response surface methodology with a three-level complete factorial design was employed to optimize these influential parameters. Therefore, five different measuring systems were utilized to construct a quadratic model and a second-order polynomial equation. Optimal levels of parameters were then obtained by analysis of the model and the numerical optimization method. When the Y. lipolytica A-101 was cultivated at optimized pH (5.0) using biofuel waste as a medium, the protein concentration was increased to 8.28—a 44% enhancement as compared to the original (3.65). This study has thus demonstrated a beneficial way to cultivate Y. lipolytica A-101 on biofuel waste for enhanced production of single cell protein and amino acids for use in human diet and in animal feed.
Highlights
Yarrowia lipolytica is one of the better-known oleaginous yeasts with the ability to grow on a variety of hydrophilic and hydrophobic substrates
Screening for optimal physical parameters for protein production The obtained biomasses of Y. lipolytica strains enriched in protein and amino acids exhibit different sensitivities to temperature and pH
Y. lipolytica growing on fatty substrates tends to metabolize poly-unsaturated fatty acids and is
Summary
Yarrowia lipolytica is one of the better-known oleaginous yeasts with the ability to grow on a variety of hydrophilic and hydrophobic substrates These can be conventional (starch, molasses, fruit, and vegetable wastes) and nonconventional (different fractions of petroleum, natural gas, ethanol, methanol, lignocellulosic biomass, waste cooking or motor oils, animal-waste fats, or waste streams from various industries). Because of such inexpenside feedstocks, microbial mass and microbial lipid produced by Yarrowia lipolytica (single cell protein and Yarrowia lipolytica is a rich natural source of various nutritional components (peptides, trace minerals, fats, especially mono-unsaturated fatty acids (MUFAs) and saturated high added-value lipids like cocoa-butter equivalents). This yeast has the ability to accumulate vitamin B12 into its cells from biofuel waste used as a medium in a similar manner to animal cells (Jach et al 2018)
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