Valorization of food waste fermentation liquid into single cell protein by photosynthetic bacteria via stimulating carbon metabolic pathway and environmental behaviour

Abstract

Single cell protein (SCP) production by photosynthetic bacteria (PSB) is dependent on the bioavailability of carbon source, while sufficient volatile fatty acids (VFAs) in food waste fermentation liquid might be a potential alternative. It is unclear how the fermentation liquid affects the SCP biosynthesis and the related metabolic mechanism. This work demonstrated that the SCP production could be improved effectively (2088.4 mg/L) with high conversion capacity of carbon source (0.99 mg-biomass/mg-COD) by regulating carbon source level. PSB preferred to utilize the VFAs in food waste fermentation liquid. The carbon metabolic pathways (e.g., the transformation of VFAs to acetyl-CoA, and tricarboxylic acid cycle) involved in the SCP production were enhanced under optimal condition. Moreover, optimal carbon source regulation could significantly stimulate the environmental behaviour of PSB (e.g., two-component system, quorum sensing, and ATP-binding cassette transporter) involved in adaptation to external stimulus and maintaining high bacterial activity, resulting in SCP yield promotion.