STABILITY OF KARYOTYPE OF COWS OF RED POLISH BREED IS TO ACTION OF EXOGENOUS OF FACTORS

Abstract

The aim of our work was comparison of changeability of karyotype of cows of Red Polish and Ukrainian Red-and-White milk breeds to the action of exogenous of factors – hydrogen sulfide in water. Applied methods: cytogenetic, test "Method of determination of mutagene activity of xenobiotics on Drosophila of melanogaster", statistical. Conducted comparative analysis of changeability of karyotype of cows of Red Polish and Ukrainian Red-and-White diary breeds to the performance of exogenous of factor – hydrogen sulfide in water showed that the animals of the Ukrainian Red-and-White diary breed the part of dinuclear lymphocytes is more than 3 times higher comparatively with the animals of the Red Polish breed with the statistically reliable difference of averages at P > 0,999. The increase of part of dinuclear lymphocytes in peripheral blood of cows of the Ukrainian Red-and-White diary breed is caused by exogenous factor (by hydrogen sulfide in water). For research of influence of water on the organism of animals the test "Method of determination of mutagene activity of xenobiotics on Drosophila of melanogaster" was conducted. The conducted test showed that at the cultures of Drosophila, grown on the environment prepared on water with the hydrogen sulfide, frequency of post-embryonic lethal mutations presented 25%. Conclusions: For the animals of the Ukrainian Red-and-White diary breed part of dinuclear lymphocytes more than 3 times higher comparatively with the animals of the Red Polish breed with the statistically reliable difference of averages at P > 0,999. The conducted test "Method of determination of mutagenesis activity of xenobiotics on Drosophila of melanogaster" showed presence of mutagenesis effect of hydrogen sulfide (χ² = 10,23; P > 0,99) but of weak activity. Increase of part of dinuclear lymphocytes in peripheral blood of cows of the Ukrainian Red-and-White diary breed, in our opinion, caused by exogenous factor (by the hydrogen sulfide in water). The cows of the Red Polish breed are characterized by a mionectic sensitiveness to the mutagene factors and more clearly expressed coadaptation processes.