STABILITY INDICATING HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METOPROLOL SUCCINATE AND TELMISARTAN

Abstract

A simple, rapid, accurate, precise, selective, and reproducible stability‐indicating HPLC method has been developed for simultaneous estimation of metoprolol succinate and telmisartan using a mobile phase consisting mixture of Methanol: 10 mM potassium dihydrogen phosphate buffer: 10 mM hexane sulphonic acid (80:10:10,v/v/v) at the flow rate of 1 mL/min and detection wavelength at 223.0 nm. HiQ Sil C18 (250 × 4.6 mm, 5 µm) column was used as stationary phase. The retention time for metoprolol succinate and telmisartan were 3.067 min and 5.653 min, respectively. Linearity was observed in the concentration range of 5–80 µg/mL for metoprolol succinate and 5–60 µg/mL for telmisartan. The coefficient of correlation for metoprolol succinate and telmisartan was found to be 0.9990 and 0.9980, respectively. The results of analysis have been validated statistically and by recovery studies. Both the drugs were subjected to acid, alkali and neutral hydrolysis, oxidation, dry heat, and photolytic degradation. The degradation products of telmisartan and metoprolol succinate were well resolved from the pure drugs with significant differences in the retention time values. This method can be successfully employed for simultaneous quantitative analysis of metoprolol succinate and telmisartan in bulk drugs and formulations.