Ssc-miR-451 Regulates Porcine Primary Adipocyte Differentiation by Targeting ACACA.

Mailin Gan,Linyuan Shen,Li Zhu,Xuewei Li,Lin Liu,Shunhua Zhang,Ya Tan,Guoqing Tang,Ye Zhao,Lei Chen,Xu Xu,Tinghuan Zhang,Yuan Fan,Qiang Li,Lili Niu

doi:10.3390/ani10101891

Abstract

Simple SummarymiR-451 has been extensively studied in humans and model animals. However, there are no reports of miR-451 in pigs, and it is unclear whether miR-451 is related to adipose tissue development. In this work, we first reported the effects of ssc-miR-451 on pig adipose development and meat quality. We focused on the expression and functions of miR-451 in porcine primary adipocyte and pork quality. Porcine primary adipocyte transfection test and dual luciferase reporting system suggested that ssc-miR-451 may inhibit lipid deposition by inhibiting Acetyl-CoA carboxylase alpha (ACACA) expression. Correlation analysis negatively correlated miR-451 expression with intramuscular fat content and positively correlated ACACA expression with intramuscular fat content. Further analysis of fatty acid composition revealed that pigs with high expression of ssc-miR-451 had higher monounsaturated fatty acid and lower polyunsaturated fatty acid.miRNA is a small non-coding RNA, which plays an important role in diverse biological processes. In the present study, we explore the effect of ssc-miR-451 on porcine adipose development and meat quality. We observed that ssc-miR-451 was downregulated during porcine primary adipocyte differentiation. Overexpression of ssc-miR-451 inhibited adipogenic differentiation, while inhibition of ssc-miR-451 promoted adipogenic differentiation. The dual luciferase reporter system indicated Acetyl-CoA carboxylase alpha (ACACA) as a target gene of ssc-miR-451. Correlation analysis negatively correlated miR-451 expression with intramuscular fat content (IMF) and positively correlated ACACA expression with IMF. Further analysis of fatty acid composition revealed that pigs with high expression of ssc-miR-451 had higher monounsaturated fatty acid (MUFA) and lower polyunsaturated fatty acid (PUFA). Taken together, our study suggests that ssc-miR-451 regulates lipid deposition and fatty acid composition by targeting ACACA, and ssc-miR-451 may serve as a potential genetic marker to improve pork quality.

Highlights

People in most countries and regions around the world have a tradition of eating pork
Compared with the control group, ssc-mir-451 over-expression significantly inhibited the expression of Fatty acid synthase (FASN), Acetyl-CoA carboxylase alpha (ACACA), and SCD1 genes, which promoted lipid synthesis
Inhibition of ssc-miR-451 significantly promoted the expression of FASN, ACACA, SCD1, and C/EBPα (Figure 2D–E)

Summary

Introduction

People in most countries and regions around the world have a tradition of eating pork. Pork quality is closely related to human health and food safety. Adipose tissue as an important component of pork is closely related to pig health, and meat quality. Adipose tissue is the main energy storage organ as well as an important metabolic and endocrine organ [1], which affects the growth and health of the pig. The fat development of pigs is closely related to the feeding time, feed consumption, and pork quality, which is directly related to the feeding cost and selling price. Adipose tissue will affect the appearance, flavor, and taste of pork. Understanding the regulation of pig fat tissue development and metabolic regulation is of great significance for pig production and disease treatment [2]

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