Splanchnic artery occlusion shock: vinblastine-induced leukopenia reduces tumour necrosis factor and thromboxane A 2 formation, and increases survival rate

Abstract

This study was designed to assess the role of leukocytes in rats subjected to splanchnic artery occlusion for 45 min followed by reperfusion (SAO shock). Leukopenia was induced by an intravenous injection of vinblastine (1 mg/kg) 72 h before SAO shock. Survival rate (within 6 h), plasma levels of thromboxane B 2 (TxB 2), serum levels of tumour necrosis factor (TNF-α), and histological alteration of the intestinal tract were investigated. Control rats [white blood cells (WBC)=10362±630/mm 3] died within 2 h following ischaemia and reperfusion. Leukopenic (WBC=1263±311/mm 3) animals which underwent SAO shock survived more than 2 h and 50% of them were still alive after 6 h. Plasma TxB 2 levels significantly increased in WBC count normal rats subjected to SAO shock (8.4±2.1 ng/ml), compared to sham animals (0.4± 0.08ng/ml); however SAO shock raised TxB 2 levels significantly less (2.1±1.1 ng/ml) in leukopenic rats. Serum TNF-α, undetectable in sham-shocked rats (either with normal WBC count or without), rose up to 150±12 U/ml in shocked rate and to 45±5 U/ml ( P<0.01) in shocked animals with leukopenia. SAO shock induced a massive necrosis of the intestinal tract in rats with normal WBC count. Leukopenia prevented ileum necrosis in SAO shock. These data indicate that leukocytes play an important role in splanchnic artery occlusion and reperfusion.