Spectroscopic, microscopic characterization of Cannabis sativa leaf extract mediated silver nanoparticles and their synergistic effect with antibiotics against human pathogen

Abstract

Silver nanoparticles (SNPs) are successfully synthesized from aqueous silver nitrate (AgNO3) through a simple novel route using the leaf extract of Cannabis sativa. The results obtained from UV–visible spectroscopy, FTIR (Fourier transform-infrared) spectroscopy, XRD (X-ray diffraction), DLS (dynamic light scattering) SEM (scanning electron microscopy), EDS (energy dispersive spectroscopy), TEM (transmission electron microscopy), SAED (selected area electron diffraction) and AFM (atomic force microscopy) indicate towards the formation of stable, spherical and crystalline SNPs in the size range of 13–25 nm with absorbance peak at 392 nm. Further, various antibiotic alone and with SNPs have been tested against different pathogenic microorganisms and the results show that control of pathogenic microorganisms is more when antibiotics are mixed with SNPs.