Abstract
The drug promethazine hydrochloride (PRZH) forms with rhodium (II) a colored chelate (?max = 472 nm) complex at (pH = 2.1) which is extractable with benzyl alcohol as organic solvent. Under the appropriate experimental conditions a calibration plot was set up from which some analytical parameter were derived and deduced by regression. Standard addition procedure was also adopted. It has been estimated that the concentration of the drug PRZH to be 24.89 mg per unit and 24.19 mg per unit for both calibrations. Under optimal conditions, the developed method has been achieved the following characteristics: LDR (30 – 150 µg ml-1 ) PRZH , RSD % ( 0.6 – 2.47 ) , sandell sensitivity( 0.0844 µg. cm -2 ) , LOD ( 1.66 µgml-1 ) , recovery % ( 100.74 ± 1.34 ) , Erel % ( 0.74 ) . Stability constant (6.4 × 10 5 M-1). The mole – ratio method (1: 1) approved that PRZH – Rh (II) as a structure of the complex. The developed procedure has been adapted to analyze PRZH in various pharmaceuticals.
Highlights
Promethazine, aphenothazine drug, is widely used as an antihistamine and a mild sedative [1].Various methods have been reported for the determination of promethazine hydrochloride these include spectrophotometry [ 2, 3, 4 ], HPLC [ 5, 6 ], capillary zone electrophoresis [7 ], titrimetry [ 8 ] .In this work, a molecular spectrophotometric method for determination of drug promethazine hydrochloride (PRZH) in some pharmaceutical preparations by chelating with Rhodium (II) has been developed
Benzyl alcohol was used as organic solvent for extraction of chelating complex
0.25 ml of (1000 μg ml –1) promethazine hydrochloride standard solution, was transferred to 5 ml volumetric flask, and diluted to the mark with water, 4 ml of this solution, was transferred to absorption cell, the absorption spectrum of this solution was measured in the region between 200 to 1100 nm using water as the reference
Summary
A molecular spectrophotometric method for determination of drug promethazine hydrochloride (PRZH) in some pharmaceutical preparations by chelating with Rhodium (II) has been developed. Extracted processes and measurement with applied as mentioned in (A) .the concentration of drug sample was obtained from the standard addition plot by regression (Fig 2). 0.25 ml of (1000 μg ml –1) promethazine hydrochloride standard solution , was transferred to 5 ml volumetric flask , and diluted to the mark with water , 4 ml of this solution , was transferred to absorption cell , the absorption spectrum of this solution was measured in the region between 200 to 1100 nm using water as the reference .
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