Abstract
A simple sensitive spectrophotometric method has been suggested for the estimation of pure p-aminophenol(p-AMPL), and p-AMPL results from the hydrolysis of paracetamol(PARL). The suggested method is based on oxidative coupling reaction of p-AMPL with 4-chlororesorcinol (4-CLRL) and potassium periodate to produce a stable, and water-soluble coloured product with maximum absorption at wavelength 556 nm. Beer's law is followed over the range of concentration from 2 to 20 µg p-AMPL. ml-1. The molar absorptivity value is equal to 1.0277×104 l.mol-1cm-1. All factors responsible for the completed reaction and highest intensity of the product have been studied, and the optimal of each factor has been selected. The suggested method was applied in an indirect method for the determination of paracetamol in tablets and injection after acidic hydrolysis to p-AMPL. The common excipients added did not interfere in the estimation of paracetamol. The suggested method was applied in an indirect method for the determination of paracetamol in tablets and injection after acidic hydrolysis to p-AMPL. The common excipients added did not interfere in the estimation of paracetamol.
Highlights
Introduction pAminophenol is an important organic compound
Acid hydrolysis of PARL Various analytical methods listed in the literature have been used in the detection and quantification assay of p-AMPL as pure or resulted from hydrolysis of paracetamol
The spectrophotometric methods included various types of reactions such as diazotization of p-aminophenol resulted from hydrolysis of paracetamol coupled with various coupling agent such as 4 [(2-amino-1, 3thiazol-4-yl) amino] nitrobenzene [12], histidine reagent in alkaline medium [13], 1-naphthol [14], 2, 7-dihydroxy naphthalene in alkaline medium [15], hydroxyl analog of the pharmaceutical naproxen [16], phloroacetophenone in an alkaline medium [17]
Summary
The determination of p-AMPL, subsequent experiments were carried out using 1 ml of 100 μg/ml pAMPL solution in a final volume of 10 ml, and the absorbance of the solution was measured at the wavelength of 556 nm versus the blank solution. This study was conducted by selecting a number of oxidizing agents such as potassium periodate, sodium nitroprusside, and N-bromosuccinimide and by adding 1 ml of each one separately to solutions containing equal concentrations of p-AMPL (10 μg/ml) in the presence of 1 ml of 0.1% of 4chlororesorcinol and a 0.5 ml of sodium hydroxide solution(1M).
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