Abstract
A spectrophotometric method based on the indophenol reaction is described for the determination of phenol in water and biological fluids. Phenol reacts with ammonia and N-chlorosuccinimide in alkaline media with sodium nitroprusside as a catalyst to give a greenish blue indophenol dye. The dye has a maximum absorption at 670 nm. Beer's law is obeyed in the range 0.3–2.4 p.p.m. The molar absorptivity and the Sandell sensitivity are 3.30 × 104 l mol–1 cm–1 and 0.0028 µg cm–2, respectively. The reaction conditions and other analytical parameters have been optimised. The method has been successfully applied to the determination of phenol in waste water and biological fluids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
