Spectrophotometric determination of folic acid using 1,10- phenanthroline materials with ninhydrin reagent

Abstract

There have been 2 spectrophotometric techniques developed, validated to find out how much folic acid there is that is simple to use, sensitive to detect, and selective. One method reduces ferric chloride in a neutral medium while simultaneously adding folic acid, and the second method is to chelate iron (II) along with 1, 10-phenanthroline (method A) on one hand and Method B depends on the interaction of the primary amino group of folic acid with the amino group of Ninhydrin in an alkaline solution, On the other hand. In Method A, red chromogens with a wavelength of 505 nm are produced, whereas, in Method B, violet chromogens with a wavelength of 575 nm are produced, and so on. Beer's law holds under ideal conditions in molar absorptivity values of 2.331104 L.mol-1 cm-1 and 6.771104 L.mol-1 cm-1, respectively, for concentration ranges of 1.0–35.0 g ml-1 and 0.5–10.0 g ml-1. Sandell's sensitivity values of 1.944 × 10–6 µg cm-2 and 1.6728 × 10–6 µg cm-2, respectively, and Sandell Technically, technique A and method B had limits of detection (LODs) of 0.198 and 0.488, respectively, and limits of quantification (LOQs) of 0.600 and 1.479, respectively. The correlation coefficients (R2) for technique A and method B were both 0.9979 and 0.9988. A and B used stability constants of 5.6192 and 5.4633 kJ.mol-1, respectively, andGibbsfree energy change (ΔG) values of −25.6071 and −24.8963, respectively, in their calculations of the free energy change (ΔG). The proposed methodologies were successfully used to evaluate folic acid in both pure and tablet form, with no influence from common excipients found in pharmaceutical formulations, demonstrating their efficacy. According to the study, there were no statistically significant differences between the precision and accuracy of the suggested procedures and those produced using the reference approach.