Spectrophotometric Assessment of Pyrocatechol Drug Employing Oxidative Coupling Reaction with 4-amino antipyrine as a Model of Pharmaceutical Compounds .

Abstract

A rapid, simple, sensitive, and higher stability spectrophotometric method was developed the determine that it is stable Pyrocatechol drug in pure and pharmaceutical preparations. The method is based on an oxidative coupling reaction among Pyrocatechol drug and 4-amino antipyrine (4AAP) in the presence of potassium iodate (KIO3) in an acid medium to form a color complex that stable violet dye and a maximum absorption at 505 nm. The best optimum conditions and other analytical factors have been evaluated. A calibration curve of absorbance of several concentrations that obey the law of Beer-Lambert over the range concentration of pyrocatechol drug 10 to 100 mg/L, with (LoD =1.2*10-4 mg/L), (LoQ=3.4*10-4 mg/L), The relative standard deviations RSD% of the proposed methods were less than 0.68%). In contrast, recovery average and determination parameters are 99.9%. The method was used successfully for the determination of drugs in a pharmaceutical preparation with the best accuracy and precision. At range about 99.1 to 100.2% with RSD less than 0.3%. The analytical result for the estimation of the pure drug is presented together with the use of the proposed process for the analysis of some commercial pharmaceuticals. The data compare favorably with those of official and reported ways.