Abstract
Simple, sensitive and accurate two methods were described for the determination of terazosin. The spectrophotometric method (A) is based on measuring the spectral absorption of the ion-pair complex formed between terazosin with eosin Y in the acetate buffer medium pH 3 at 545 nm. Method (B) is based on the quantitative quenching effect of terazosin on the native fluorescence of Eosin Y at the pH 3. The quenching of the fluorescence of Eosin Y was measured at 556 nm after excitation at 345 nm. The two methods obeyed Beer’s law over the concentration ranges of 0.1-8 and 0.05-7 µg/mL for method A and B respectively. Both methods succeeded in the determination of terazosin in its tablets
Highlights
Terazosin 1-(4-Amino-6,7-dimethoxy-2-quinazolinyl)-4-[(tetrahydro-2-furanyl) carbonyl]piperazine hydrochloride (Fig.1) are one of group drugs called alpha-adrenergic blockers
It facilitates the passage of blood pass through veins and arteries by relaxation of smooth muscle in blood vessels. It relaxes the muscles in the prostate and bladder neck, improving the urinary flow. It was indicated for the treatment of hypertension, or to improve urination in men with benign prostatic hyperplasia[1,2]
The spectrophotometric method included the addition of increasing amounts 0.1-8 μg/mL of standard terazosin solution transferred into set of 10 mL standard volumetric flasks 1.5 mL of 5×104 M eosinY solution and 1 ml of acetate buffer were added to each flask followed by adjustment to volume with distilled water and the absorbance value was measured against blank solution at 545 nm
Summary
Piperazine hydrochloride (Fig.1) are one of group drugs called alpha-adrenergic blockers It facilitates the passage of blood pass through veins and arteries by relaxation of smooth muscle in blood vessels. Analytical literature review shows a number of methods to determine trerazosin in pharmaceutical preparations and biological fluids such as: reversed phase high performance liquid chromatography, HPLC [3,4,5], high performance thin layer chromatography, HPTLC [4,6], Electrochemical [7,8,9,10,11]. The aim from this work is the development of two methods That are simple, accurate, sensitive and fast utilizing spectrophotometric and spectrofluorimetric and applying to pharmaceutical preparations using the eosineY dye. The present methods do not need to control the temperature or extraction process
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