Sonochemically N-functionalized graphene oxide towards optically active photoluminescent bioscaffold.

Abstract

Herein, Nitrogen functionalized graphene oxide (N-f-GrO) has been synthesized using the sonochemical method. 2-Aminopyrimidine (APD) was used as a precursor for covalent functionalization with graphene oxide [f-(APD)GrO] as N-f-GrO which was ascertained with XPS. The involvement of arylamine group and formation of covalent bond over GrO surface was confirmed with high resolution C1s spectrum of f-(APD)GrO. Also, the signature of N1s peak in the survey spectrum of f-(APD)GrO has endorsed the surface modification of GrO through covalent functionalization. A bathochromic shift was observed for f-(APD)GrO in UV and enhanced weight loss of 91.39% at 191.80 °C, confirms a facile functionalization of GrO via formation of amide bond, where the terminal -OH portal of carboxylic group is substituted by 2-Aminopyrimidine. Moreover, the formation of f-(APD)GrO was investigated with various analytical techniques like Raman, XRD and FTIR. The surface morphology and topography have been understood by using HRTEM/SAED, AFM, and SEM analysis. The synthesized f-(APD)GrO shows potential optically active photoluminescence properties and higher potency towards biological insight. The identified photoluminescence (PL) peaks at 3.78, 3.21 2.01 and 1.64 eV indicate photon emission including an orange optical transition at 2.01 eV. The multiple peaks in a PL spectrum are due to radiative and non-radiative recombinations which are also associated with excess hole (h+)-electron (e-) trapping on the surface to restrict the recombinations of e- and h+. The biological activity of N-f-GrO has been explored with Sulforhodamine B (SRB) assay on HaCaT and Vero cell lines. The concentration-dependent cell viabilities have been observed a maximum at 20 µg/ml for HaCaT and at 10 µg/ml for Vero cell lines at testing concentration range of 10-80 μg mL-1. The significant morphological impact on cell lines confirms the cytocompatibility behaviour. Therefore, the synergistic impact of various properties of f-(APD)GrO can be further explored to study its significance as nanocarrier for photosensitive biomedical response.