Solid Phase Analytical Derivatization

Abstract

Sample preparation method is crucial in analytical chemistry and especially for the complicated matrices encountered in biological and environmental samples. The challenge in sample preparation in such analysis is the recovery of analytes in an isolate suitable for determination at high sensitivity and specificity. Solid phase analytical derivatization (SPAD) combines attractive features of both solid phase extraction and analytical derivatization. As such it provides increase in sensitivity, specificity of detection for selected functional groups, low organic solvent consumption, ease of automation, high throughput and better separation in chromatography. In SPAD analytes are extracted onto a solid phase and derivatized in one single step. Over the years many SPAD based methods have been reported in literature using various combinations of solid phases, analytical derivatization reagents and detectors.