SOLID LIPID NANOPARTICLE: FABRICATED THROUGH NANOPRECIPITATION AND THEIR PHYSICOCHEMICAL CHARACTERIZATION

Arun Sharma,Arun Parashar,Vineet Mehta,Udayabanu Malairaman,Riddhi Patrwal

doi:10.22159/ijpps.2016v8i10.13207

Abstract

Objective: Aim of the present study was to formulate solid lipid nanoparticles (SLNs) and to determine their physicochemical parameters when stored at cold temperature in aqueous solution (D-SLNs) prior to biological application.Methods: SLNs were formulated though nanoprecipitation technique which comprised of stearic acid (lipid), poloxamer 188 and lecithin (surfactant). Physicochemical parameters were estimated though particle size analysis, polydispersity index, surface morphology analysis (Scanning electron microscopy and Transmission electron microscopy) and cytotoxicity studies followed by live-dead staining through acridine orange and ethidium bromide.Results: SLNs with spherical morphology were successfully fabricated as revealed though SEM and TEM investigations. Fabricated SLNs had the mean particle size ranging from 188 nm (SLNs) to 327 nm (D-SLNs). Zeta potential was found to be±14mV to±6mV and polydispersity index was 0.297±0.18 for SLNs without incubation and 0.538±0.07 for SLNs after incubation. No cytotoxicity was observed for SLNs.Conclusion: SEM and TEM investigations showed morphological variation in SLNs and D-SLNs. Dissimilarity in mean particle size, zeta potential and polydispersity index indicates the increase in size and aggregation of nanoparticles. No cytotoxic effects of SLNs were observed in normal cells, suggesting storage of nanoformulation in the aqueous state has no effect in context to cytotoxicity. Hence we conclude that prolonged storage of formulation at cold temperature causes the deterioration of polymeric formulation.

Highlights

In recent past, biological applications in nanotechnology have gained much consideration from scientific world to develop new advanced nanoparticle system for better therapeutic applications [1]
SEM and Transmission electron microscopy (TEM) are complimentary to each other and enable us to recognize the morphological changes in formulations by quantitatively observing the particle size and shape
solid lipid nanoparticles (SLNs) were fabricated by nanoprecipitation and auxiliary investigation were carried out to evaluate various physicochemical parameters during prolonged storage of lipid nanoformulation

Summary

Introduction

Biological applications in nanotechnology have gained much consideration from scientific world to develop new advanced nanoparticle system for better therapeutic applications [1]. The lipid-based system is the best instance of nano-technological advancements in this perspective, which has undergone extensive research in past decade [2, 3]. These lipid-based formulations are well acknowledged as solid lipid nanoparticles (SLNs) which in general are solid lipid-based matrix systems. Prolonged storage of nanoformulation in aqueous solution at cold storage (4 °C) leads to deformity or development of cracks on the surface of the nanosphere, which changes physicochemical characteristics and leads to failure of the formulation [13] This problem can be eliminated successfully by lyophilisation, but its higher cost and increased chances of getting contaminated limits its use [14]

Methods

Results

Conclusion