SİNİR BÜYÜME FAKTÖRÜ İLE FARKLILAŞTIRILMIŞ PC12 HÜCRELERİNİN MORFOMETRİK VE FLORESANS ANALİZİ

Abstract

Objective
 PC12 is a rat pheochromocytoma cell line. These
 cells characteristically undergo differentiation when
 cultured with nerve growth factor (NGF). Depending
 on the dose of NGF, the length of neurite extensions
 changes. Thanks to this differentiation property,
 the cells are used in neuroscience and in modeling
 pathophysiological diseases such as Alzheimer's,
 Parkinson's, and Amyotrophic Lateral Sclerosis.
 However, literature studies showing the effect of NGF
 on neurite extensions formed in PC12 cells are very
 limited. This study aimed to investigate the effect of
 NGF on neurite extensions and cell viability depending
 on dose and incubation time.
 Materials and Methods
 In this study, PC12 cells were incubated with 50 ng/ml
 and 100 ng/ml NGF for 3, 6 and 7 days. The lengths
 of neurite outgrowths and dead cell ratios were
 calculated in incubated cells.
 Results
 The results showed that the length of neurite
 extensions and dead cell ratio increased depending
 on NGF doses and incubation time. When NGF
 incubation times were compared, no difference was
 found between 50 ng/ml NGF 6 days and 100 ng/ml
 NGF 3 days groups.
 Conclusion
 When the dead cell ratios and sizes of neurite
 extensions in the experimental groups are evaluated,
 it is thought that 100 ng/ml NGF and 3 days incubation
 time parameters are ideal for PC12 cell differentiation.