Skuteczność działania olejku lawendowego (Oleum Lavandulae) wobec grzybów drożdżopodobnych z rodzaju Candida

Abstract

Introduction. A number of plants produced of essentials oils, which can utilize in medicine. Oleum Lavandulae (Lavender oil) demonstrated antimicrobial effect connected with presence of components such as linalool, linalyl acetate, lavandulol, ocimene, terpinen-4-ol, cyneol, lavandulol acetate, α- i β-pinene, camphen and camphor. Several studies have demonstrated antifungal activity towards filamentous fungi, dermatophytes and some genus of yeastlike fungi. Aim. The aim of the study was determined activity of lavandulae oil towards yeastlike fungi, from Candida species. Material and methods. A total 44 strains of yeastlike fungi isolated from oral cavity from patients with candidosis and 9 reference strains were tested. The susceptibility of Candida strains was determined by means plate dilution technique in Sabouraud’s agar. The inoculums contained 105 CFU per spot were seeded with Steers replicator upon the agar plate containing lavender oil and without lavender oil (the strains growth control). Incubation the agar plates was performed in aerobic conditions at 37°C for 24-48 hrs. The MIC was defined as the lowest concentrations of oil that completely inhibited growth of tested yeastlike fungi. Results. The results showed, that all tested fungi were susceptible in concentrations 1.0-≥ 2.0 mg/ml. The most susceptible to lavender oil were strains from the genus Candida guilliermondii, C. lusitaniae and C. utilis. The growth of this fungi was inhibited by concentrations = 1.0 mg/ml. The 8 (38%) strains belonging to the genus of C. albicans were sensitive on 1.0 mg/ml. The growth of remaining strains was inhibited by concentrations ≥ 2.0 mg/ml. The strain of C. humicola was the lowest sensitive (MIC > 2.0 mg/ml). The tested lavender oil characterized a high activity toward date yeastlike fungi. The growth 50% of the strains was inhibited by concentrations of 1.0 mg/ml and others 48% by 2.0 mg/ml. Conclusions. Lavender oil showed high activity against tested strains of yeastlike fungi. The strains from genus C. guilliermondii, C. lusitaniae and C. utilis were the most susceptible. The oil was the lowest active to the strain from genus C. humicola.