Abstract
Haepyoyijin-tang (HPYJT) is a traditional herbal prescription that is composed of 12 medicinal herbs. Although HPYJT is frequently used in patients with asthma in Korea, no quality assessment protocols have been developed. In the present study, qualitative and quantitative analyses were performed using high-performance liquid chromatography and liquid chromatography–tandem mass spectrometry on the eight main components (mulberroside A, amygalin, liquiritin apioside, liquiritin, narirutin, hesperidin, rosmarinic acid, and glycyrrhizinic acid) to establish a quality control protocol for HPYJT. The simultaneous analysis method developed was satisfactorily validated with respect to linearity, limit of detection, limit of quantification, recovery, and precision. This analytical method thus provides an efficient approach for the evaluation of HPYJT quality.
Highlights
Haepyoyijin-tang (HPYJT) is an herbal medicine prescription that consists of 12 medicinal herbs
HPYJT has been demonstrated to exhibit biological action, such as protective effects on pulmonary injury caused by SO2, and to affect airway mucin production and gene expression control in respiratory diseases that are accompanied by mucus hypersecretion [2,3]
To establish an efficient quality control protocol for HPYJT, we developed and validated the first simultaneous quantitative analysis using high-performance liquid chromatography equipped with photodiode array detector (HPLC–PDA) system on the eight main constituents of HPYJT
Summary
Haepyoyijin-tang (HPYJT) is an herbal medicine prescription that consists of 12 medicinal herbs Pericarpium [5], steroids (e.g., ergosterol peroxide), sesquiterpenoids (e.g., (S)-(+)-turmerone), and triterpenes (e.g., pachymic acid and polyporenic acid C) from Poria Sclerotium [6], flavonoids (e.g., liquiritin and liquiritin apioside) and triterpenes (e.g., glycyrrhizinic acid and 18β-glycyrrhizinic acid) from Glycyrrhizae Radix et Rhizoma [7], phenolic compounds (e.g., rosemary acid and elemicin), monoterpenes (e.g., perillaldehyde) from Perillae Folium [8], alkaloids (e.g., ephedrine and pseudoephedrine) form Ephedrae Herba [9], phenolic compounds (e.g., amygdalin and neoamygdalin) from Armeniacae Semen [10], phenolic compounds (e.g., mulberroside A) and flavonoids From Mori Radicis Cortex, amygdalin (AMY) from Armeniacae Semen, liquiritin apioside (LIQA), liquiritin (LIQ), and glycyrrhizinic acid (GA) from Glycyrrhizae Radix et Rhizoma, hesperidin (HES). Narirutin (NAR) from Citri Unshius Pericarpium, and rosmarinic acid (RA) from Perillae Folium
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