Abstract
The Sogunjung decoction (SGJD) is a traditional herbal formula that has been used to treat constipation and improve the constitution of infirm children in Korea. In this study, simultaneous quantification of gallic acid (1), magnoflorine (2), albiflorin (3), paeoniflorin (4), liquiritin apioside (5), liquiritin (6), liquiritigenin (7), coumarin (8), cinnamaldehyde (9), benzoylpaeoniflorin (10), and glycyrrhizin (11) was conducted using fast and sensitive liquid chromatography–tandem mass spectrometry (LC–MS) multiple-reaction monitoring to develop a quality-control protocol for the SGJD. A Waters Acquity UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) was used for the chromatographic separation of the 11 marker compounds in the SGJD using two mobile phases (5 mM ammonium acetate in distilled water containing 0.1% (v/v) formic acid, and acetonitrile). The MS parameters for a simultaneous analysis were capillary voltage (3.0 kV), source temperature (150 °C), desolvation temperature (500 °C), desolvation gas flow (700 L/h), and cone gas flow (50 L/h). The developed LC–MS method was validated by the evaluation of linearity, limits of detection, limits of quantification, recovery and precision. By using the developed and validated assay, the 11 marker components in the SGJD were detected in amounts of 0.01–51.83 mg/g.
Highlights
Traditional Korean medicine (TKM), traditional Chinese medicine (TCM), and Kampo medicine (KM) have been widely used in Asian countries such as Korea, China, and Japan to enhance human health and prevent various diseases
This study focused on simultaneous quantification for quality control of the Sogunjung decoction (SGJD) using high-performance liquid chromatography (HPLC)–photodiode array detector (PDA)
The present research focused on the simultaneous analysis of 11 marker compounds using liquid chromatography–tandem mass spectrometry (LC–MS) in a multiple-reaction monitoring (MRM) mode: gallic acid (1), albiflorin (3), paeoniflorin (4), and benzoylpaeoniflorin (9) from P. lactiflora, coumarin (8) and cinnamaldehyde (10) from C. cassia, liquiritin apioside (5), liquiritin (6), liquiritigenin (7), and glycyrrhizin (11) from G. uralensis, and magnoflorine (2) from Z. jujube
Summary
Traditional Korean medicine (TKM), traditional Chinese medicine (TCM), and Kampo medicine (KM) have been widely used in Asian countries such as Korea, China, and Japan to enhance human health and prevent various diseases They are traditionally prescribed in the form of a combination of two or more herbal medicines based on a traditional medicine philosophy and have mostly been used as a decoction [1,2]. The present research focused on the simultaneous analysis of 11 marker compounds using LC–MS in a multiple-reaction monitoring (MRM) mode: gallic acid (1), albiflorin (3), paeoniflorin (4), and benzoylpaeoniflorin (9) from P. lactiflora, coumarin (8) and cinnamaldehyde (10) from C. cassia, liquiritin apioside (5), liquiritin (6), liquiritigenin (7), and glycyrrhizin (11) from G. uralensis, and magnoflorine (2) from Z. jujube
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