Abstract
Background: The degree of human hepatocyte replacement inchimeric mice with humanized liver has previously been shown to correlate with human plasma albumin measurements. However, there are no reportsthatdirectly comparetheremaining endogenous mouse albumin with the newly expressed human albumin following engraftment. To better understand the disposition of serum albumin in PXB-mice, we developed aliquid chromatographytandem mass spectrometry (LC-MS/MS) method to simultaneously quantitate both human and mouse albumin from plasma. Results: A robust correlation was observed between the serum human albumin levels measured by LC-MS/MS and the estimated replacement index of PXB-mice. Conclusion: All data wereshown to be specific and suitable to accurately quantify both human and mouse albumin from plasma of chimeric mice with humanized livers.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call