Abstract
The H-point standard addition method (HPSAM) is applied to kinetic data for the simultaneous determination of ascorbic acid (AA) and L-cysteine (Cys). If present in excess amounts, iron(III) is quantitatively reduced to iron(II) by AA or Cys which in turn, in the presence of phenanthroline (phen), produces a colored iron(II)-phen complex showing maximum absorbance at 510 nm. HPSAM is based on the difference of the reaction rates between AA and Cys with Fe(III). The linear dynamic ranges for the two analytes AA and Cys are 0.50–4.00 ppm and 1.00–8.00 ppm, respectively. The RSD% for 6 replications of AA and Cys at 3.00 ppm is 2.30 and 2.70, respectively. The predictability of the method is checked by determining the two analytes in real samples. The various effects of several similar reducing agents are investigated.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
