Simultaneous determination of uric acid and dopamine using a carbon fiber electrode modified by layer-by-layer assembly of graphene and gold nanoparticles

Jiao Du,Zhangquan Yao,Ruirui Yue,Ping Yang,Yukou Du,Fangfang Ren,Fengxing Jiang

doi:10.1007/s13404-013-0090-0

Jiao Du, Zhangquan Yao + Show 5 more

Open Access

https://doi.org/10.1007/s13404-013-0090-0

Copy DOI

Journal: Gold Bulletin	Publication Date: May 19, 2013
Citations: 93	License type: CC BY 2.0

Affiliation: Soochow University

Abstract

A novel layer-by-layer assembly of graphene sheets and gold nanoparticles modified carbon fiber electrode (GE/Au/GE/CFE) was successfully fabricated and applied to simultaneous determination of dopamine (DA) and uric acid (UA). The structure of GE/Au/GE/CFE was characterized by scanning electron microscopy (SEM). It was observed that the gold nanoparticles were homogeneously assembled between the two layers of GE sheets. Cyclic voltammetry (CV) measurements elucidate that GE/Au/GE/CFE has higher electrocatalytic activity for the oxidation of DA and UA when compared with graphene modified carbon fiber electrode (GE/CFE), and graphene and gold nanoparticles modified carbon fiber electrode (Au/GE/CFE). Simultaneous determination of UA and DA on GE/Au/GE/CFE was conducted with a differential pulse voltammetry technique, and two well-defined and fully resolved anodic oxidation peaks were observed. Anodic oxidation currents of DA and UA are linear with their concentration in the range of 0.59–43.96 μM and 12.6–413.62 μM, respectively. Moreover, the composite electrode displays high reproducibility and selectivity for the determination of UA and DA.

Highlights

Dopamine (DA), one of the most important catecholamine neurotransmitters, exists in the mammalian central nervous system at very low concentrations and plays a significantJ
The image shows that gold nanoparticles are well distributed to form a nanocomposite on the surface of GE sheets
Compared with the Cyclic voltammetry (CV) response of graphene modified carbon fiber electrode (GE/CFE), an obvious increase in the redox peak current and a narrow peak potential difference are observed in the CV response of Au/GE/CFE, suggesting that the introduction of gold nanoparticles can enhance the electrochemical activity of obtained electrode

Summary

Introduction

Dopamine (DA), one of the most important catecholamine neurotransmitters, exists in the mammalian central nervous system at very low concentrations and plays a significantJ. Li et al [25] developed GE and gold nanoparticles modified electrodes which can selectively determine DA with a high detection limit (1.86 μM).

Results

Conclusion