Simultaneous Determination of Four Ingredients in Plantago Depressa by Single Marker

Shengnan Bi,Yang Xu,Yanxue Jia,Huiwei Bao,Silvia Persichilli,Yuejie Wang

doi:10.1155/2021/4040239

Abstract

Objective. To establish a quantitative analysis of multicomponents by single marker (QAMS) method for the simultaneous determination of 4 active components such as protocatechuic acid, catechin, quercetin, and luteolin in Plantago depressa. Method. 4 active components in Plantago depressa were studied. Quercetin was used as an internal reference to establish the relative correction factors among protocatechuic acid, catechin, and luteolin and calculate the contents of each component; the results were compared with those measured by the external standard method. Results. 4 components showed a good linear relationship in their respective concentration ranges (r > 0.9995). The relative correction factors (fs/k) of protocatechuic acid, catechin, and luteolin were 1.1992, 0.8613, and 1.6069, respectively. The method had good durability. The contents of protocatechuic acid, catechin, and luteolin calculated by QAMS were not significantly different from those measured by the external standard method. Conclusion. QAMS can be used to determine the content of 4 components in Plantago depressa at the same time, and the method is simple, accurate, and can be used for quality control.

Highlights

Plantago depressa is recorded in the Chinese Pharmacopoeia (2015 edition) [1], which is the dried whole plant of Plantago asiatica L. or Plantago depressa Wild., with the effects of clearing heat, promoting diuresis, relieving stranguria, eliminating phlegm, cooling blood, and detoxifying
According to the literature on the quality standard of Plantago depressa, the single component is mainly used as the evaluation index of the quality of Plantago depressa, which is contradictory to the characteristics of the multicomponent synergistic effect of traditional Chinese medicine
In the previous studies of this subject, it was found that protocatechuic acid, catechin, quercetin, and luteolin contained in Plantago depressa had not been reported for content evaluation, and these four components had remarkable effects in antibacterial activity [8,9,10,11,12,13], detumescence, anti-inflammatory [10,11,12,13], eliminating phlegm, antivirus [11], and protecting the liver and gallbladder [14,15,16], which indicates that it is of great significance to detect the contents of protocatechuic acid, catechin, quercetin, and luteolin in Plantago depressa

Summary

Introduction

Plantago depressa is recorded in the Chinese Pharmacopoeia (2015 edition) [1], which is the dried whole plant of Plantago asiatica L. or Plantago depressa Wild., with the effects of clearing heat, promoting diuresis, relieving stranguria, eliminating phlegm, cooling blood, and detoxifying. It can be used for treating heat stranguria, difficulty and pain, edema, oliguria, summer humidity, alvine flux, phlegm, heat cough, carbuncles, etc. Plantago depressa has a long history, and it is widely used in China Whether it is a single medicinal material or a compound preparation [2], it has a certain clinical application market. In the previous studies of this subject, it was found that protocatechuic acid, catechin, quercetin, and luteolin contained in Plantago depressa had not been reported for content evaluation, and these four components had remarkable effects in antibacterial activity [8,9,10,11,12,13], detumescence, anti-inflammatory [10,11,12,13], eliminating phlegm, antivirus [11], and protecting the liver and gallbladder [14,15,16], which indicates that it is of great significance to detect the contents of protocatechuic acid, catechin, quercetin, and luteolin in Plantago depressa

Methods

Results

Conclusion