Abstract
Objective. To develop a quantitative analysis of multicomponents by single-marker (QAMS) method for the simultaneous determination of polar active components in Fructus Corni. Methods. Loganin was selected as the internal reference, and the relative correction factors (RCFs) of gallic acid, 5-hydroxymethyl-2-furfural, morroniside, sweroside, cornin, 7α-O-methylmorroniside, 7β-O-methylmorroniside, 7α-O-ethylmorroniside, 7β-O-ethylmorroniside, and cornuside were established. The contents of multicomponents were then calculated based on their RCFs, respectively. Contents of the 11 components were also calculated by external standard method and compared with those of the QAMS method. Results. The contents of the 11 components in 21 crude and 10 processed Fructus Corni products were measured. No significant difference was found in the quantitative results of the QAMS and external standard methods. Conclusion. QAMS could serve as an accurate and convenient method in determining the polar and active components in Fructus Corni and its processed products.
Highlights
Fructus Corni is derived from the dried ripe sarcocarp of Cornus officinalis Sieb. et Zucc (Cornaceae)
The results showed that, by choosing loganin as the internal reference substance and establishing the relative correction factors (RCFs) of other 10 components, this method proved reliable, accurate, and simple for determining those active components in Fructus Corni and its processed products
The purity for each reference compound was greater than 98% as validated by High-performance liquid chromatography (HPLC) analysis. 7α-O-Methylmorroniside, 7β-O-methylmorroniside, 7α-O-ethylmorroniside, and 7β-O-ethylmorroniside were prepared by our lab and structurally characterized based on 1H-NMR and 13C-NMR spectra
Summary
Fructus Corni is derived from the dried ripe sarcocarp of Cornus officinalis Sieb. et Zucc (Cornaceae). In the practice of traditional medicine, Fructus Corni has a role in nourishing the liver and kidney, which provides the pharmacological basis for its wide use in many herbal prescriptions [1]. Modern researches have shown that Fructus Corni has many chemical constituents, such as volatile constituents, iridoids, organic acids, carbohydrates, and tannins [2]. Iridoid glycosides are a family of active ingredients in Fructus Corni, which are reported to have many pharmacological activities including immune regulation, glycemic lowering, anticoagulation, antiarrhythmia, neuroprotection, and memory improving [3,4,5]. Gallic acid and 5-hydroxymethyl-2-furfural, which belong to organic acids, are reported to contribute to the effect of Fructus Corni in improving liver and kidney functions and related antioxidative effects [6, 7]
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