Abstract
A HPLC method has been developed for simultaneously detecting chlorogenic acid, ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, ligustilide, and levistolide A in Angelicae Sinensis Radix through quantitative analysis of multicomponents by single-marker (QAMS) method with ferulic acid as internal standard substance. The relative analysis correction factors of each component in Angelicae Sinensis Radix have good reproducibility under different chromatography conditions. In addition, no significant difference of results was found between quantitative analysis of multicomponents by single-marker (QAMS) method and external standard method in determining content of these components of different Angelicae Sinensis Radix and its 12 kinds of preparations. As a result, the established QAMS method for Angelicae Sinensis Radix analysis with ferulic acid as internal standard substance is accurate and feasible, which could be used as an effective and economical method to control quality of Angelicae Sinensis Radix and its herbal products.
Highlights
Angelicae Sinensis Radix (ASR, named Danggui in Chinese) is the dried root of Angelica sinensis (Oliv.) Diels [1]
The simultaneous determination of multicomponents in ASR is mainly concentrated on ferulic acid, ligustilide, and senkyunolide I through using high performance liquid chromatography (HPLC). It could not make a comprehensive and scientific evaluation of ASR and its herbal product [25, 26]. erefore, the quantitative analysis of multicomponents by single-marker (QAMS) was used to simultaneously determine ferulic acid, chlorogenic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, ligustilide, and levistolide A in this study, the ferulic acid was used as internal standard substance, and the relative calibration factors (RCF) of other compounds was established. e established method could simultaneously determine the contents of 8 active components (Figure 1) in ASR and its 12 kinds of preparations, which provides a theoretical scientific basis for the total quality control and evaluation of ASR and its preparations
Ferulic acid (≥98%) was purchased from SAIN Chemical Technology Co., Ltd. (Shanghai, China), chlorogenic acid, ligustilide, senkyunolide A, SenkyunolideI, and levistolide A were purchased from Chengdu Pfeide Biotechnology Co., Ltd. (Chendu China) with purity ≥98%, senkyunolide H and coniferyl ferulate were purchased from Sichuan Dexter Biological Co., Ltd. (Sichuan China) with purity ≥98%; and 10 batches of ASR were bought from Yellow River Medicinal Material Market of Gansu Province (Gansu China), which was identified by associate professor Daiyu Qiu (Department of Traditional Chinese Medicine, Gansu Agricultural University)
Summary
Angelicae Sinensis Radix (ASR, named Danggui in Chinese) is the dried root of Angelica sinensis (Oliv.) Diels [1]. E method used a cheap and available component as an internal standard substance to establish the relative calibration factors (RCF) between the internal standard substance and other components that are to be tested so that the simultaneous determination of multiple components to be tested can be realized To some extent, this method could reduce the analysis cost of quality control of TCM [22,23,24]. The simultaneous determination of multicomponents in ASR is mainly concentrated on ferulic acid, ligustilide, and senkyunolide I through using high performance liquid chromatography (HPLC) It could not make a comprehensive and scientific evaluation of ASR and its herbal product [25, 26]. It could not make a comprehensive and scientific evaluation of ASR and its herbal product [25, 26]. erefore, the QAMS was used to simultaneously determine ferulic acid, chlorogenic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, ligustilide, and levistolide A in this study, the ferulic acid was used as internal standard substance, and the RCF of other compounds was established. e established method could simultaneously determine the contents of 8 active components (Figure 1) in ASR and its 12 kinds of preparations, which provides a theoretical scientific basis for the total quality control and evaluation of ASR and its preparations
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