SIMULTANEOUS DETERMINATION OF AMILORIDE HYDROCHLORIDE AND HYDROCHLOROTHIAZIDE IN PHARMACEUTICALS BY DERIVATIVE SPECTROPHOTOMETRY

Abstract

Method is established for simultaneous determination of Amiloride hydrochloride (Amilo) and hydrochlorothiazide in tablets. The method is based on employing normal and derivative spectrophotometry using the zero order via described by measuring the absorbance at 361 nm and the first order derivative spectra at peak 340 and valley 382 nm for (Amilo), while the zerocrossing method is applied for hydrochlorothiazide. The first order derivative absorption spectra at valleys 285 and 239 nm were used for hydrochlorothiazide. No interferences found between both determined and those of matrices. A good accuracy and precision of simultaneous determination of (Amilo), and hydrochlorothiazide were confirmed by statistical analysis. The percentage recovery of the individual drags under the established conditions is ranged from 97.79% to 101.5 %. Linearity is maintained within a wide concentration range from 2.00 mg/L to 40.00 mg/L for Amiloride hydrochloride and hydrochlorothiazide. The detection limit was 0.5 and 1.0 mg/L for Amiloride hydrochloride and hydrochlorothiazide respectively.