Abstract
Derivatives spectrophotometric techniques were developed for the determination of Amoxicillin Trihydrate (Amox) with Potassium Clavulanate (PC) antibiotic binary mixtures. The simultaneous determination of these compounds was accomplished by derivative (1D, 2D and 3D) spectrophotometric technique and applying zero-crossing technique used for determination of (Amox) and (PC) in tablets. The second order derivative absorption spectra at valley λ=299 nm were used for (Amox) and also the second order derivative spectra at peak λ=239.5 nm were used for (PC). No interferences were found between both determined constituents and those of matrix. A good accuracy and precision of simultaneous determination of (Amox) and (PC) were confirmed by statistical analysis. The recovery of individual constituents under established conditions is very high and ranges for synthetic standards mixture and tablets from 100.11, 99.33 and 96.98, 96.84 respectively. Linearity is maintained within a wide concentration range from 2.0 to 90.0 μg.mL-1 and from 10.0 to 90.0 μg.mL-1 for (Amox) and (PC) and linearity percentage 99.98 and 99.99 respectively. The detection limit is 0.211 μg.mL-1 for (Amox) and 0.259 μg.mL-1 for (PC). The corresponding quantitation limits are 0.704 μg.mL-1 (Amox) and 0.864 μg.mL-1 for (PC).
Highlights
Potassium clavulanate (PC), as shown in figure (1-a) is the salt of clavulanic acid that belongs to the β-lactamase inhibitors
Amoxicillin Trihydrate with Potassium Clavulanate Mixture Figure (1-a) shows the normal spectrum cannot be used to determine each of Amoxicillin trihydrate (Amox) and PC present in their mixture, due to interfering between the spectra, ; derivative spectrophotomeric methods 1D, 2D, 3D can be used in this case Figure (1b,c, d)
Amox can be determined at P = 267.0, V = 283.5 nm, on the other hand, PC can be determined at V = 229.0 nm
Summary
Potassium clavulanate (PC), as shown in figure (1-a) is the salt of clavulanic acid that belongs to the β-lactamase inhibitors. Potassium clavulanate occurs as a white to light yellowish white, crystalline powder, hygroscopic. It is freely soluble in water, slightly soluble in alcohol, very slightly soluble in acetone [1]. Several different methods have been used for determination of potassium clavulanate including; High-performance liquid chromatographic [2,3,4,5,6]. Almost white crystalline powder, slightly soluble in water and in alcohol, practically insoluble in ether and in fatty oils. It dissolves in dilute acids and dilute solutions of alkali hydroxides.
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