Spectrophotometric Methods for the Estimation of Isoxsuprine Hydrochloride in Bulk and Oral Dosage Form

Abstract

Four new, simple, sensitive and reproducible spectrophotometric methods have been developed for the estimation of isoxsuprine hydrochloride in tablet dosage form. Method A involves the determination of isoxsuprine hydrochloride by Q-absorbance ratio method at 269nm and 274nm; the Beer's concentration range was found to be 20-100 µg/mL. Method B and Method C involves the determination of isoxsuprine hydrochloride by first derivative spectrophotometry and second derivative spectrophotometry respectively. The normal spectrum was derivatized to first and second order derivative spectrum and the linearity was found to lie within the Beer's range for isoxsuprine hydrochloride. Method D involves the determination of isoxsuprine hydrochloride by Area under curve method (AUC) and the linearity was established. The correlation coefficient for all the four methods were found to be 0.999 and the developed methods were analyzed for specificity, limit of detection (LOD), limit of quantification (LOQ), linearity of response, precision and accuracy. Thus the proposed methods could be adopted for routine analysis of bulk drug and its formulation. Keywords: Area under curve (AUC), Beer's law, Derivative Spectrophotometry, Isoxsuprine hydrochloride, Q-Absorbance ratio