Abstract
This study describes an inexpensive, simple and green method to form silver nanoparticles from different leaf extracts of Achyranthes aspera and Scoparia dulcis plants. The silver nitrate is reduced by Achyranthes aspera and Scoparia dulcis leaf extracts respectively to generate two silver nanoparticle types symbolized as AA.AgNPs and SD.AgNPs. The optical absorption, size and morphology of silver nanoparticles are significantly impacted by extract types. The ultraviolet visible spectrum of AA.AgNPs shows a 433-nm peak being more broadened than that of SD.AgNPs. The Fourier infrared transform spectra of two of these silver nanoparticles revealed that their surface is modified by organic constituents from extracts, and thus they are stabilized in solution without any additional reaction. Images from transmission electron microscopy and scanning electron microscope indicate that AA.AgNPs are in clusters with the size of 8–52 nm almost possessing oval shape, while SD.AgNPs are smaller size of 5-45 nm separated well in diversified shapes (spherical, triangle, quadrilateral and hexagonal). Moreover, both AA.AgNPs and SD.AgNPs exhibit the highly antifungal effect against Aspergillus niger, Aspergillus flavus and the most strong impact on Fusarium oxysporum. For these obtained results, two new silver nanoparticles are promising fungicides for various applications of medical and agricultural fields.
Highlights
For a long time, the irregular use of fungicides threatened plants, animals and human health and led to the extreme consequence of antifungal resistance
All of the experiments were used with deionized water (DIW) filtrated by water purification systems (Milli-Q HX 7150, Merck Millipore, Guyancourt, France)
An ecofriendly synthesis of Aspergillus niger producing extracellularly silver nanoparticles (AgNPs) using A. aspera and S. dulcis leaf extract was successfully performed. This method provided an alternative approach for silver nanoparticle
Summary
The irregular use of fungicides threatened plants, animals and human health and led to the extreme consequence of antifungal resistance. This problem has been becoming more seriously, the mortality rates related to fungal infection, especially antifungal resistance. The biosynthesized nanoparticles are formed by an ecofriendly technique or green chemistry. This method utilized less energy, minimized the toxic chemicals, simplified the procedure and exploited the natural materials being able to regenerate [11]. The green synthesis of nanoparticles has been more attractive than the physical and chemical methods
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