Abstract
Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial blight in rice, which reduces crop yield and leads to significant economic losses. Bacterial sigma (σ) factors are highly specialized proteins that allow RNA polymerase to recognize and bind to specific promoters. σ70 factors also regulate the expression of genes involved in stress response and virulence. However, the role of RpoD in Xoo is still unclear. In this study, we found that σ70 factor RpoD is quite conservative among phytopathogenic bacteria, especially in Xanthomonas sp. In Xoo, PXO_RpoD plays an important role in oxidative stress tolerance and cell motility, as well as being essential for full virulence. Cleavage under targets and tagmentation (CUT&Tag) analyses indicated that RpoD mediates the type three secretion system (T3SS) by regulating the regulation of hrpG and hrpX. By performing bacterial one-hybrid and electrophoretic mobility assay (EMSA), we observed that RpoD directly bound to the promoters of hrpG and hrpX. Collectively, these results demonstrate the transcriptional mechanism and pathogenic functions of RpoD in regulating cell motility and oxidative stress response, providing novel insights into potential targets for disease control.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
