Abstract
Guan, Z.-j., Guo, B., Huo, Y.-l., Guan, Z.-p., Dai, J.-k. and Wei, Y.-h. 2012. Short Communication: Organogenesis and somatic embryogenesis in callus derived from HBsAg-transgenic tomato mutant. Can. J. Plant Sci. 92: 747–756. In the process of hepatitis B surface antigen (HBsAg) gene transformation, a transgenic cherry tomato (Lycopersivon esculentum Mill.) mutant N244 was occasionally obtained. Based on its obvious variation in ploidy and phenotypic characteristics, morphogenesis and development in the mutant in vitro were studied by cytological analysis and ultrastructural examination. The adventitious shoot primordia first emerged in the mutant N244 leaf explants after 14 d of culture, and the somatic embryo pathway developed after 35 d. Transmission electron microscopy and scanning electron microscopy further indicated that during the explant's development, there were significant changes in morphology and quantity of some organelles in N244 callus cells. On the 7th day of culture, there was little cy...
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