Somatic Embryogenesis of Rosa Hybrida L.

Abstract

ABSTRACTWe present two regeneration protocols via somatic embryogenesis in three cut roses—Rosa hybrida cvs. “Anny”, “Trimontsium” and “Saltze Gold”. The first regeneration protocols are based on regeneration via somatic embryogenesis in callus from leaf and petiole segments of Rosa hybrida cvs. “Trimontsium” and “Saltze Gold”. The other protocol is via somatic embryogenesis in callus culture from petals of Rosa hybrida cv. “Anny”. Both regeneration protocols were developed on solid medium under subculturing on every 35–40 days. MS-medium including MS (1962—Murashige & Skoog) or QL (Quorin & Lepoivre) vitamins were used. For the embryo induction of callus derived from leaf and petiole explants were used BAP, NAA and kinetin, while for the combination of dicamba, kinetin and 2-ip are the most efficient for the embryo induction of callus derived from petals. A part of these embryos was converted to intact plants and an other part had been multiplicated and maintained in vitro for two years in order to check their regeneration capacity. All the regenerants that had been obtained from the three cut roses were adapted under in vitro conditions and cultivated in a greenhouse. Regenerated plantlets were morphologically true to the type of the initial donor-genotype.