Shoot regeneration from hormone-autonomous callus from shoot cultures of several sugarbeet ( Beta vulgaris L.) genotypes

Abstract

Shoot cultures of six sugarbeet genotypes produced callus when grown in dim fluorescent light (10–15 μEm −2 s −1) at 30°C on Murashige-Skoog (MS) medium with 0.25 mg/l 6-benzyladenine (BA). Hormone autonomy of the callus was indicated by sustained callus growth following multiple transfers onto MS medium lacking auxin and cytokinin. Shoots were regenerated from 1-month-old callus of two genotypes after transfer to MS medium with 0.25–5.0 mg/l BA at 30°C in dim fluorescent light (10–15 μEm −2 s −1).