Abstract
Accumulative evidence has shown the adverse effects of a geomagnetic field shielded condition, so called a hypomagnetic field (HMF), on the metabolic processes and oxidative stress in animals and cells. However, the underlying mechanism remains unclear. In this study, we evaluate the role of HMF on the regulation of cellular reactive oxygen species (ROS) in human neuroblastoma SH-SY5Y cells. We found that HMF exposure led to ROS decrease, and that restoring the decrease by additional H2O2 rescued the HMF-enhanced cell proliferation. The measurements on ROS related indexes, including total anti-oxidant capacity, H2O2 and superoxide anion levels, and superoxide dismutase (SOD) activity and expression, indicated that the HMF reduced H2O2 production and inhibited the activity of CuZn-SOD. Moreover, the HMF accelerated the denaturation of CuZn-SOD as well as enhanced aggregation of CuZn-SOD protein, in vitro. Our findings indicate that CuZn-SOD is able to response to the HMF stress and suggest it a mediator of the HMF effect.
Highlights
Living organisms were exposed in the geomagnetic field (GMF) throughout the evolutionary history
In the non-synchronized cells, the cellular reactive oxygen species (ROS) in the day 2 hypomagnetic field (HMF)-exposed cells was lower (P = 0.001, HMF vs. GMF; One-way ANOVA Bonferroni post hoc test) than that in the control, and the reduction was at the same level with that in cells treated with 1 mmol/L NAC (2-day) (P = 0.007, NAC vs. GMF; P = 0.059, HMF vs. NAC; One-way ANOVA Bonferroni post hoc test), an antioxidant reagent which can reduce cellular ROS and led to ∼30% increase in cell proliferation as that in the HMF groups (P = 0.022, NAC vs. GMF; P = 0.002, HMF vs. GMF; P = 0.617, HMF vs. NAC; One-way ANOVA Bonferroni post hoc test)
The results suggest that the HMF accelerates the proliferation of SH-SY5Y cell by reducing cellular ROS level
Summary
Living organisms were exposed in the geomagnetic field (GMF) throughout the evolutionary history. It has been established that that a GMF-shielded condition, so called a hypomagnetic field (HMF) (Mo et al, 2012), adversely affect many aspects of the living system, e.g. embryonic development (Mo et al, 2012; Wan et al, 2014; Osipenko et al, 2008; Fesenko et al, 2010), animal behaviors (Bliss and Heppner, 1976; Prato et al, 2005; Zamoshchina et al, 2012; Mo et al, 2015), and brain function (Zhang et al, 2004; Xiao et al, 2009; Binhi and Sarimov, 2009). The adverse physiological effects of the HMF has raised concerns on the.
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