Abstract
Accumulating evidence has shown that absence of the geomagnetic field (GMF), the so-called hypomagnetic field (HMF) environment, alters the biological functions in seemingly non-magnetosensitive cells and organisms, which indicates that the GMF could be sensed by non-iron-rich and non-photo-sensing cells. The underlying mechanisms of the HMF effects on those cells are closely related to their GMF sensation but remain poorly understood so far. Previously, we found that the HMF represses expressions of genes associated with cell migration and cytoskeleton assembly in human neuroblastoma cells (SH-SY5Y cell line). Here, we measured the HMF-induced changes on cell morphology, adhesion, motility and actin cytoskeleton in SH-SY5Y cells. The HMF inhibited cell adhesion and migration accompanied with a reduction in cellular F-actin amount. Moreover, following exposure to the HMF, the number of cell processes was reduced and cells were smaller in size and more round in shape. Furthermore, disordered kinetics of actin assembly in vitro were observed during exposure to the HMF, as evidenced by the presence of granule and meshed products. These results indicate that elimination of the GMF affects assembly of the motility-related actin cytoskeleton, and suggest that F-actin is a target of HMF exposure and probably a mediator of GMF sensation.
Highlights
Reported the negative effects of the hypomagnetic field (HMF) on the central nervous system (CNS), which include changed vocal behaviour in birds[24]; perturbed circadian rhythm in birds and humans[25,26,27,28]; and inhibition of stress-induced analgesia[28,29,30], decreased noradrenaline release[29,31,32], impaired learning and memory[33,34], and weakened visual performance in humans[35]
HMF pre-exposure significantly reduced (p = 0.048) cells’ adhesive capacities to the BSA blank bottom, as compared to the geomagnetic field (GMF) control. This difference was not observed in the groups in which the adhesive coatings were used, indicating that the strength of the adhesive coating materials masked the inhibitory effect of the HMF on cell adhesion (Fig. 2a)
After 48 h incubation in the HMF environment, a greater number of retracted and blunt cell protrusions were observed by F-actin staining, as well as fewer F-actin filopodia at the cell edge, when compared to cells incubated in the GMF (Fig. 6a,b)
Summary
Wei-Chuan Mo1,2, Zi-Jian Zhang[1,3], Dong-Liang Wang[1], Ying Liu[1], Perry F. Disordered kinetics of actin assembly in vitro were observed during exposure to the HMF, as evidenced by the presence of granule and meshed products These results indicate that elimination of the GMF affects assembly of the motility-related actin cytoskeleton, and suggest that F-actin is a target of HMF exposure and probably a mediator of GMF sensation. Disordered kinetics of actin assembly in vitro was observed during exposure to the HMF, which was provided by a Helmholtz coils system[40] (Fig. 1b) These results suggest that elimination of the GMF affects the assembly of the motility-related actin cytoskeleton, and that the F-actin cytoskeleton responses to the HMF exposure and probably a mediator in cells’ GMF sensation
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