Serum Colloidal Reactdon and Liver Function Test in Cattle—Evaluation of Serum Colloidal Reaction by Multivariate Analysis

Abstract

Organic information obtained from blood is generally more useful for liver function tests than any other information. At present, many items of these tests can be estimated by using particular kits or micro methods. There is, however, a wide range of the normal value of each of them in animals on account of feeding environment, age, and pregnance. Therefore, it is difficult to make the diagnosis of a given disease on the basis of a small change in value of a given item of the test.The serum colloidal reaction can detect changes in protein metabolism in the liver sensibly. It does not require any complicated instrument, but clarifies the condition of liver function by estimating several items of the test in a short time. To evaluate this reaction in a more objective manner, 57 serum samples were subjected to such four types of the reaction, ZTT, TTT, Gross' reaction, and Lugol's reaction, as had been performed rather commonly. They were also subjected to biochemical tests to determine the following 12 items: total protein, albumin, inorganic phosphete, total cholesterol, glucose, blood urea nitrogen, uric acid, creatinine, Al-P, GOT, GPT, and LDH. Moreover, the protein fractionation was performed on them by electrophoresis. Then, statistical analysis was made to elucidate to what extent the serum colloidal reaction reflected the condition of the liver. At the same time comparison was made between macroscopical autopsy findings of the liver and the results of the serum colloidal reaction.As a result, the four types of this reaction, except TTT, showed a high correlation with the r-globulin level and such hepatic changes as atrophy, hypertrophy, and cholangitis. In conclusion, the usefulness of the serum colloidal reaction was demonstrated from the results of analysis by the statistical method.