Abstract
A gas-liquid chromatographic (GLC) method adapted from Schmit and Mater is described for the measurement of total and free cholesterol in serum. The method has been found to be simple, sensitive and precise. The results have been compared with the colorimetric method of Carr and Drekter for total cholesterol and the method of Sperry and Webb for free cholesterol. For total cholesterol lower values were obtained by the GLC method both in sera from patients without signs of liver disease and in sera with high total bilirubin. There was a tendency for somewhat higher values for free cholesterol by the GLC method than by the colorimetric method. This difference was not significant. The present study has demonstrated that the GLC method for total and free cholesterol determination is more specific than colorimetric methods. Gas-liquid chromatography should be given consideration as a reference method for the estimation of free cholesterol and total cholesterol.
Published Version
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