Abstract
Seven of the selected bile acids were investigated: cholic acid (C), chenodeoxycholic acid (CDC), deoxycholic acid (DC), lithocholic acid (LC), glycocholic acid (GC), glycodeoxycholic acid (GDC), and glycolithocholic acid (GLC). The retardation factor (R F ), ΔR F values, the separation factors (α), resolution factors (R S ), as well as the constants of the pair separation (R F α), indicate that the mobile phase n‐heptane–ethyl acetate–acetic acid about volume composition 25:20:8, is the best for separations of investigated bile acids on aluminum plates precoated silica gel 60 F254.
Published Version
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