Abstract
The aim of our study was to determine the lipophilicity of selected bile acids, i.e., cholic acid (C), glycocholic acid (GC), glycodeoxycholic acid (GDC), chenodeoxycholic (CDC), deoxycholic acid (DC), lithocholic acid (LC), and glycolithocholic acid (GLC) with the use of reversed phase thin‐layer chromatography on CNF254 plates (E. Merck, #1.12571) and methanol‐water, organic mixture (acetonitrile–methanol 50∶50, v/v)–water, acetone–water, dioxane–water in different volume compositions as mobile phases. Lipophilicities RMW and ϕ0 were compared, both with measured partition coefficients (logPexp) and the calculated ones (AlogPS, IAlogP, logPKOWIN, xlogP, clogP, logPRekker). The most significant correlation was found between RMW and ϕ0 lipophilic parameters and logPKOWIN values. Moreover, it was stated that the obtained parameters RMW and ϕ0 correlate best with experimental partition coefficients (logPexp) given after Roda and coauthors. The values of RMW and ϕ0 lipophilic parameters obtained on RP18W, RP2, and CNF254 plates with the use of the a/m mobile phases, indicate that the investigated bile acids may be listed in order of decreasing lipophilicity as follows: LC>DC≈CDC≈GLC>C≈GDC>GC.
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