Separation of neutral carbohydrates by capillary electrophoresis

Abstract

The basic strategies for analysis of neutral carbohydrates by capillary electrophoresis are summarized. Neutral carbohydrates are dissociated in strong alkali to give anions, hence they can be separated directly by zone electrophoresis based on the difference between their dissociation constants. However, neutral carbohydrates are not electrically charged under normal conditions. Therefore, they should be converted to ions prior to or during analysis. Precapillary introduction of a basic or an acidic group to a neutral carbohydrate gives the derivative positive (in acidic media) or negative (in alkaline media) charge, respectively. The derivatives thus obtained can be separated by zone electrophoresis. Analysis of carbohydrates in a carrier containing an oxyacid salt (such as sodium borate) or an alkaline metal salt (such as calcium acetate) causes in situ conversion to anionic or cationic complexes, respectively, which are separated by zone electrophoresis. The effective uses of electrokinetic chromatography in sodium dodecyl sulfate micelles for hydrophobic derivatives (such as 1-phenyl-3-methyl-5-pyrazolone derivatives) and size-exclusion electrophoresis in gel-packed capillaris for size different oligosaccharides are also discussed. Each separation mode has its inherent method(s) for detection, which are also described here.