Abstract
Publisher Summary This chapter discusses the use of two electrically driven microcolumn separation techniques in the analysis of carbohydrates: capillary electrophoresis (CE) and capillary electrochromatography (CEC). While CE has made significant progress in the area of carbohydrate analysis, the potentials of CEC have started to be exploited in the separation of carbohydrates. There are additional merits and sound features for using both CE and CEC in the analysis of carbohydrates as complementary to each other or as stand-alone analytical separation techniques. Similar to high-performance liquid chromatography (HPLC) and polyacrylamide gel electrophoresis (PAGE), CE and CEC are also aqueous-based separation methods well suited for the analysis of inherently hydrophilic compounds, such as carbohydrates. CE and CEC possess several advantages over HPLC and PAGE in that they (1) provide higher separation efficiencies, (2) yield shorter analysis time, (3) require small sample amounts, and (4) consume lower amounts of expensive reagents and solvents.
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