Abstract
The migration of iodothyronines through Sephadex columns is greatly influenced by the pH and ionic strength of the eluents. By controlling these variables, a method has been developed for separating the iodo compounds of serum into four discrete fractions: iodoprotein, iodide, triiodothyronine and thyroxine, during a single passage through a column of Sephadex G-25. The method is relatively rapid (less than 3 h per column run), and cam be employed with large samples of serum. Recovery of labeled iodothyronines added to serum is essentially complete. Chromatography of purified labeled compounds showed less than 0.2% deiodination; no conversion of thyroxine to triiodothyronine during the procedure could be demonstrated.
Published Version
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