Abstract
Cellulose acetate zone electrophoretic analyses of foot-and-mouth disease virus (FMDV), type A, strain 119, with different passage histories revealed differences in homogeneity and response to chymotrypsin induced mobility changes. Foot-and-mouth disease virus with a high passage history in calf kidney cells migrated as a single electrophoretic component at the pH and ionic strengths examined. This virus, purified after a single additional passage in baby hamster kidney cells, clone 13, also gave a single zone. In contrast, virus purified after a single passage in uncloned baby hamster kidney cells revealed two electrophoretic zones, 1 and 2, as determined by staining and infectivity. Zone 1 migrated the same distance as the single virus zone prepared from the high passage calf kidney cells. All FMDV with low passage histories had only a single electrophoretic zone.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call