Abstract
The most widely used technique for separating abnormal haemoglobins as Hb F, Hb S and Hb A2 is cellulose acetate or citrate agar electrophoresis [4]. However, none of these methods gives satisfactory results in the quantitation of abnormal haemoglobins at low percentage levels. Ion-exchange chromatography has allowed considerable refinement of the determination of abnormal haemoglobins [3] and is in common use especially for quantitation of non-enzymatically glycated haemoglobin HbA1 c as useful index of long-term control in diabetes mellitus. More rapid chromatographic methods have been using highperformance liquid chromatography (HPLC) with the ion-exchange resin Bio-Rex 70 [2] or fast protein liquid chromatography (FPLC) using a monodisperse cation exchanger Mono S (Pharmacia) [1]. The aim of this study was to develop a method by which all haemoglobin variants of clinical importance could be separated and quantitated in one system.
Published Version
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