Sensitivity of parodontopatogens to the cell juice of Siberian fir (microbiological investigation)

Abstract

BACKGROUND: Search for new effective means of preventing inflammatory complications in the state of peri-implant tissues is relevant; in particular, products from coniferous greens produced by the Solagift company (Tomsk) are of interest. The bioeffective cell juice of the Siberian fir, the 100% carbon dioxide extract of Siberian fir (water fraction), stands out among them. Fir cell juice is recommended for enhancing immunity, preventing inflammatory diseases of the upper respiratory tract, and treating several pathological conditions. Fir juice contains vitamin C, carotene, polyphenolic compounds, flavonoids, iron, magnesium, copper, manganese, zinc, and maltoliron chelate complex (a natural antioxidant).
 AIM: This microbiological study aimed to examine the sensitivity of periodontal pathogens to bioeffective cell juice of Siberian fir of various concentrations.
 MATERIAL AND METHODS: A number of periodontal pathogens were cultivated in the presence of bioeffective Siberian fir cell juice in the following proportions: 1:5, 1:10, and 1:15. Incubation for up to 3 days was conducted in a reverse-spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density (OD) of the culture at a wavelength () of 850 nm. OD was measured in McFarland units (mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontal pathogens: adaptive (lag phase), exponential (log phase), stationary, and withering away. The following clinical isolates of microorganisms were used: Streptococcus constellatus, Staphylococcus aureus, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans.
 RESULTS: The microbiological study of the sensitivity of periodontal pathogens (such as S. constellatus, S. aureus, F. nucleatum, and A. actinomycetemcomitans) to bioeffective cell juice of Siberian fir showed a growth slowdown of microorganisms in the presence of fir juice and inhibition of the growth of a culture of periodontal pathogens when co-cultivated with fir juice. A significant decrease in the OD of cultures of all studied periodontal pathogens was observed at a fir juice concentration of 1:10 and 1: 5. At these concentrations, there was no growth of S. aureus culture, and the degree of decrease in the average OD of other periodontal pathogens reached 27.1% (A. actinomycetemcomitans), 31.8% (F. nucleatum), and 33.6% (S. constellatus).