СТЕПЕНЬ ПОДАВЛЕНИЯ ГРИБКОВОЙ ФЛОРЫ РТА ПРИ ИСПОЛЬЗОВАНИИ МАСЛЯНО-ЭФИРНОГО КОМПЛЕКСА ИЗ ХВОИ ПИХТЫ

Abstract

The main cause of peri-implantitis is the activity of periodontal pathogens with insufficient oral hygiene, which causes disintegration of intraosseous dental implants against the background of chronic inflammation in peri-implant tissues. The correct tactics of the post-prosthetic stage in patients with dental implants is medical examination with regular professional oral hygiene, which is complicated by low patient compliance. In this regard, there are high requirements for the level of individual oral hygiene and for the effectiveness of local hygiene products, among which dental rinses occupy an important place. As rinses, it is possible to use pine-containing substances, in particular, a complex of oil-essential fir of Siberian production of the company “Solagift” (Tomsk). The purpose of the study: microbiological study of the sensitivity of periodontopathogens and C. albicans fungi to the oil-essential complex of Siberian fir of different concentrations. Material and methods: a number of periodontopathogens and C. albicans were cultured in the presence of a complex of oil-essential Siberian fir in the following proportions 1:5, 1:10, 1:15. Incubation lasting up to three days was carried out in the Reverse Spinner RTS-1 bioreactor (BioSan, Latvia) with automatic analysis of the optical density of the culture (OD) at a wavelength of λ = 850 nm. The optical density was measured in McFarland units (Mcf). The assessment of culture growth control was based on the analysis of the growth phases of periodontopathogens: adaptive (lag phase), exponential (log phase), stationary, dying. The following clinical isolates of microorganisms were used: Streptococcus constellatus; Staphylococcus aureus; Fusobacterium nucleatum; Aggregatibacter actinomycetemcomitans, as well as Candida albicans. Results of the study: Joint cultivation of periodontopathogens with the oil-ether complex of Siberian fir reduces the optical density of cultures of clinical isolates when breeding coniferous substance 1:15 – 1:5 by 13.7% – 27.1% (A. actinomycetemcomitans), 18.3% – 62.0% (F. nucleatum), 30.0% – 56.4% (S. aureus), 19.2% – 74.1% (S. constellatus). The studied coniferous complex suppresses the culture of C. albicans at a concentration of 1:5, reducing the optical density of the culture of fungi in the microbiological experiment by 29.8%